Detection of Ubiquitylated Proteins in Mammalian Cells
- Corresponding author (tansey{at}cshl.edu)
INTRODUCTION
This assay is performed to detect ubiquitylated proteins in transfected mammalian cells. Twenty-four to 40 hours after transfection, cells are harvested, total ubiquitylated proteins are recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting.
