Imaging Protein Interactions by FRET Microscopy: Labeling Proteins with Fluorescent Dyes
This protocol was adapted from “Imaging Protein Interactions by FRET Microscopy,” Chapter 32, in Protein-Protein Interactions (eds. Golemis and Adams). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.INTRODUCTION
This protocol provides a method for labeling proteins, such as antibodies and purified recombinant proteins, with succinimide esters of sulfoindocyanine (Cy) dyes. Cy dyes covalently bind to free amino groups (the α-amino-terminal or ϵ-amino groups on lysine side chains).
