Imaging Protein Interactions by FRET Microscopy: Cell Preparation for FRET Analysis
This protocol was adapted from “Imaging Protein Interactions by FRET Microscopy,” Chapter 32, in Protein-Protein Interactions (eds. Golemis and Adams). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.INTRODUCTION
The following protocol describes the preparation of cells for FRET analysis on live and fixed cells. The reagents used have been optimized to minimize the quenching of GFP mutants and fluorescent dyes.
