Recipe

Buffer A for ubiquitylation

143.3 g guanidine HCl

25 ml Na2HPO4/NaH2PO4 (1 M)

2.5 ml imidazole (1 M)

Adjust pH to 8.0 with NaOH, and bring the final volume to 250 ml with H2O. The final concentrations should be 6 M guanidine HCl, 0.1 M Na2HPO4/NaH2PO4, and 10 mM imidazole.

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  1. doi:10.1101/pdb.rec10750 Cold Spring Harb Protoc 2006: pdb.rec10750-
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