Recipe

TI buffer

5 ml Tris-Cl (1 M, pH 6.8)

4 ml imidazole (1 M)

Adjust pH to 6.8 and volume to 200 ml with H2O. Final concentrations are 25 mM Tris-Cl and 20 mM imidazole.

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  1. doi:10.1101/pdb.rec10751 Cold Spring Harb Protoc 2006: pdb.rec10751-
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