Table of Contents

Protocols

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  6-Azauracil Sensitivity Assay for Yeast

  • William P. Tansey

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4613
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  Freeze-Thaw Lysis for Extraction of Proteins from Mammalian Cells

  • William P. Tansey

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4614
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  Detection of Ubiquitylated Proteins in Yeast

  • William P. Tansey

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4615
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  Constructing and Expressing GFP Fusion Proteins

  • David L. Spector and
  • Robert D. Goldman

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4649
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  Plating Cells for Microinjection

  • David A. Dean

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4650
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  Preparation (Pulling) of Needles for Gene Delivery by Microinjection

  • David A. Dean

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4651
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  DNA Preparation and Needle Loading for Gene Delivery by Microinjection

  • David A. Dean

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4652
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  Gene Delivery by Direct Injection (Microinjection) Using a Pulsed-Flow System

  • David A. Dean

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4653
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  Gene Delivery by Direct Injection (Microinjection) Using a Controlled-Flow System

  • David A. Dean

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4654
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  Silver Staining, Digestion, and Extraction of Peptides from an Acrylamide Gel for MS Analysis

  • Sherry Niessen,
  • Ian Mcleod,
  • and John R. Yates III

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4661
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  Direct Enzymatic Digestion of Protein Complexes for MS Analysis

  • Sherry Niessen,
  • Ian Mcleod,
  • and John R. Yates III

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4662
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  HPLC Separation of Digested Proteins and Preparation for Matrix-Assisted Laser Desorption/Ionization Analysis

  • Sherry Niessen,
  • Ian Mcleod,
  • and John R. Yates III

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4663
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  Transformation of Agrobacterium Using Electroporation

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4665
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  Transformation of Agrobacterium Using the Freeze-Thaw Method

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4666
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  PCR Analysis of Agrobacterium

  • Phil Wigge

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4667
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  In Planta Transformation of Arabidopsis

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4668
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  Kanamycin Selection of Transformed Arabidopsis

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4669
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  Glufosinate Ammonium Selection of Transformed Arabidopsis

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4670
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  Root Transformation of Arabidopsis

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4671
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