DNA Preparation and Needle Loading for Gene Delivery by Microinjection
This protocol was adapted from “Gene Delivery by Direct Injection and Facilitation of Expression by Mechanical Stretch,” Chapter 4, in Live Cell Imaging (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.INTRODUCTION
For microinjection into cells, high-quality plasmid DNA is purified using standard procedures. The resulting preparation is then delivered into the microinjection needle by either a back-filling or a forward-filling approach.
