Root-inducing medium (RIM)
1X MS (Murashige and Skoog) salts
10 g sucrose
0.5 g 2-[N-morpholino]ethanesulfonic acid (MES)
Agarose
1 ml Gamborg’s B5 vitamins (1000X)
1 ml α-naphthylphthalamic acid (NAA) (1000X)
500 mg timentin (optional, see below)
500 mg carbenicillin may be used in place of timentin
For 1 liter RIM, add MS salts, sucrose, and MES. Adjust pH to 5.7 with KOH, and add agarose to 0.5% (w/v). After autoclaving, cool medium to ~60ºC and then add Gamborg’s B5 vitamins and NAA (final concentration 2 mg/liter). If there are problems with Agrobacterium growth, use RIM with 500 mg/liter timentin or carbenicillin.
