Nondenaturing Protein Immunoprecipitation from Mammalian Cells
- Corresponding author (tansey{at}cshl.edu)
INTRODUCTION
This protocol describes a nondenaturing immunoprecipitation (IP) for mammalian cells. Where possible, we prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. However, the nondenaturing protocol is useful when one wishes to separate soluble from insoluble proteins, or when the antibody being used recognizes a native epitope.
