Protocol

Pulse-Chase Assay for Measuring Protein Stability in Yeast

  1. William P. Tansey
  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
  1. Corresponding author (tansey{at}cshl.edu)

INTRODUCTION

This assay directly measures the stability of a specific protein in yeast. Following pulse-labeling of yeast cells, the target protein is recovered by denaturing immunoprecipitation.

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  1. doi:10.1101/pdb.prot4641 Cold Spring Harb Protoc 2007: pdb.prot4641-
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