Denaturing Protein Immunoprecipitation from Yeast
- Corresponding author (tansey{at}cshl.edu)
INTRODUCTION
Where possible, it is preferable to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background and simplifies protein harvest. Yeast are boiled in an SDS-containing solution to liberate total proteins. This is also much safer than bead-beating radioactive yeast samples.
