Data Analysis Methods for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)
- Paul R. Selvin1,
- Tyler Lougheed,
- Melinda Tonks Hoffman,
- Hyokeun Park,
- Hamza Balci,
- Benjamin H. Blehm and
- Erdal Toprak
- ↵1Corresponding author (selvin{at}uiuc.edu)
INTRODUCTION
FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. Data analysis of a FIONA experiment requires the use of several software programs. Data must be acquired and exported into a proper format before analysis can take place. This article describes various options for data analysis.
