Recipe

Brain culture medium

50% (v/v) modified Eagle’s medium (MEM)

25% (v/v) HBSS (GIBCO-BRL)

25% (v/v) horse serum

2 mM glutamine

0.044% (w/v) NaHCO3

10 U/mL penicillin-streptomycin

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  1. doi:10.1101/pdb.rec11151 Cold Spring Harb Protoc 2007: pdb.rec11151-
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