Recipe

TIRFM low-calcium buffer

120 mM NaCl

2.5 mM KCl

0.5 mM CaCl2

1.25 mM EGTA

1 mM MgCl2

10 mM HEPES (pH 7.2)

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  1. doi:10.1101/pdb.rec11161 Cold Spring Harb Protoc 2007: pdb.rec11161-
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