Recipe

Filter culture medium (FCM)

Reagent Final concentration
Minimal essential medium (without phenol red) 50% (v/v)
Hank’s balanced salt solution 25% (v/v)
Horse serum, heat-inactivated 25% (v/v)
NaHCO3 0.044% (w/v)
Glutamine (or glutaMAX) 2 mM
Penicillin/streptomycin 10 U/mL

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  1. doi:10.1101/pdb.rec11167 Cold Spring Harb Protoc 2007: pdb.rec11167-
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