Recipe

FIONA imaging buffer with β-mercaptoethanol

Reagent Quantity
10% (w/v) glucose 4 μL
1 M MgCl2 1 μL
T50 buffer (or similar) 93 μL
β-mercaptoethanol 1 μL
Gloxy 1 μL
Add the gloxy just prior to using the imaging buffer. The final volume is 100 μL.

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  1. doi:10.1101/pdb.rec11170 Cold Spring Harb Protoc 2007: pdb.rec11170-

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