Recipe

FIONA imaging buffer with Trolox

Reagent Quantity
10% glucose 4 μL
1 M MgCl2 1 μL
Trolox solution (saturated) 94 μL
Gloxy 1 μL
Add the Gloxy just prior to using the imaging buffer. The final volume is 100 μL.
At high concentrations, Trolox can interfere with motility; therefore, 40% Trolox is recommended for motility experiments. To prepare 40% Trolox, decrease Trolox in the recipe above to 40 μL, and add:
54 μL of T50 buffer, or
54 μL of M5 buffer (for myosin assays).

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  1. doi:10.1101/pdb.rec11173 Cold Spring Harb Protoc 2007: pdb.rec11173-
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