Recipe

Trizma reduction buffer

Trizma base (0.2 M, pH 8.4)

Dithiothreitol (DTT) (10 mM)

EDTA (2 mM)

Add DTT just before using this buffer.

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  1. doi:10.1101/pdb.rec10738 Cold Spring Harb Protoc 2007: pdb.rec10738-
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