Recipe

Salts (10X)

Reagent Final concentration
NaCl 3 M
EDTA 50 mM
Na2HPO4 100 mM
Tris-Cl (pH 6.8) 0.1 M
Ficoll 400 (Sigma, F4375) 0.2% (w/v)
Polyvinylpyrrolidone (Sigma, PVP360) 0.2% (w/v)
BSA (fraction V, Sigma, A7906) 0.2% (w/v)
Store in aliquots at −20°C.

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  1. doi:10.1101/pdb.rec11107 Cold Spring Harb Protoc 2007: pdb.rec11107-
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