Recipe

Hybridization mix

Reagents Final concentration
SSC 1.3X
EDTA (pH 8.0) 5 mM
Formamide 50% (v/v)
CHAPS 0.5% (w/v)
Heparin 100 μg/mL
Tween 20 0.2%
Yeast RNA 50 μg/mL
The hybridization mix can be stored at −20°C.

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  1. doi:10.1101/pdb.rec11176 Cold Spring Harb Protoc 2007: pdb.rec11176-
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