Recipe

NP-40 cell lysis buffer

10 mM Tris-Cl (pH 7.4)

10 mM NaCl

3 mM MgCl2

0.5% NP-40 (Nonidet P-40)

0.15 mM spermine

0.5 mM spermidine

Store at 4ºC.

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  1. doi:10.1101/pdb.rec11188 Cold Spring Harb Protoc 2007: pdb.rec11188-
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