Recipe

MNase-Southern hybridization solution

Reagent Amount to add (per membrane)
3 mg/mL sonicated denatured salmon sperm DNA 0.4 mL
H2O 4.6 mL
10% (w/v) SDS 1.2 mL
40% sodium dextran sulfate 3 mL
20X Denhardt’s 0.6 mL
20X SSC 2.4 mL
Make fresh just before using.

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  1. doi:10.1101/pdb.rec11193 Cold Spring Harb Protoc 2007: pdb.rec11193-
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