PB1

Recipe

Reagent	Final concentration
NaCl	8.0 g/L
KCl	0.2 g/L
Na₂HPO₄ • 12H₂O	2.88 g/L
KH₂PO₄	0.2 g/L
CaCl₂ • 2H₂O	0.13 g/L
MgCl₂ • 6H₂O	0.1 g/L
Sodium pyruvate for PB1	0.036 g/L
Phenol red-bicarbonate solution for PB1	0.01 g/L
Penicillin for PB1	0.06 g/L
Glucose	1.0 g/L
Bovine serum albumin	4.0 g/L
Combine the reagents in the order listed. The pH should be between 7.3 and 7.4, and the osmolarity between 286 and 292 mOsm/L. Sterilize the solution by passing it through a 0.22-μm filter. Store it in 50-mL aliquots at 4ºC for up to 2 wk.