Recipe

Nucleic acid staining solution (NASS) (pH 4.8)

Reagent Amount to add Final concentration
Phosphate-citrate buffer tablets (Sigma-Aldrich) Two tablets 0.1 M
Disodium EDTA (Sigma-Aldrich) 0.18 g 5 mM
NaCl (Sigma-Aldrich) 0.9 g 0.15 M
Bovine serum albumin, fraction V (Sigma-Aldrich) 0.5 g 0.5% (w/v)
Saponin (Sigma-Aldrich) 0.02 g 0.02% (w/v)
To prepare 100 mL of NASS (pH 4.8), dissolve two tablets of phosphate-citrate buffer in 100 mL of H2O, and then add the remaining reagents. Store at 4°C.

| Table of Contents

This Article

  1. doi:10.1101/pdb.rec11225 Cold Spring Harb Protoc 2007: pdb.rec11225-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue