Table

Table 2. Expression vectors for MCP-xFP fusion proteins

Vector MCP-fusion Promoter Species Selection Reference
pMS2-GFP-NLS MCP-GFP-NLS pol II Mammalian Kan/Neo Fusco et al. (2003)
GFP-MS2-1xNLS GFP-MCP-1xNLS CMV Mammalian Kan/Neo Dynes and Steward (2007)
bNLS-GFP-MS2 bNLS-GFP-MCPa CMV Mammalian Kan/Neo Dynes and Steward (2007)
K89 GFP-NLS-MCP CMV Mammalian Kan/Neo Bi et al. (2003)
pCaS-Phsp83-MCP-GFP/RFP-tubulin 3′UTR NLS-MCP-GFP/RFP with the α-tubulin 3′UTR Hsp83 Drosophila Forrest and Gavis (2003); Weil et al. (2006)
pDEXH 82 MS2-GFP MCP-GFP Dictyostelium Actin 15 Dictyostelium Blasticidin Chubb et al. (2006)
pG14-MS2-GFP NLS-MCP-GFP GDP S. cerevisiae ampR, LEU, 2 μm Bertrand et al. (1998)
pCP-GFP GFP-MCP MET25 S. cerevisiae ampR, HIS, CEN Beach et al. (1999)
aBitartate NLS from Xenopus nucleoplasmin protein

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  1. doi:10.1101/pdb.tab2top28 Cold Spring Harb Protoc 2007: pdb.tab2top28-

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