Table 3. Automated microscope imaging system components

	Microscope system feature	Examples of equipment (see Fig. 3)	Manufacturer
1	Inverted microscope stand	IX81	Olympus
2	Vibration isolation table	63-500 series laboratory table	Technical Manufacturing Corporation
3	Ultrasensitive camera	Cascade II 512 EMCCD (16 μm/pixel)	Photometrics
4	Axial position controller	MS-2000-XYLE-PZ	Applied Scientific Instruments Inc.
5	Tunable excitation light source	Lambda DG4/OF30	Sutter Instruments, Inc.
6	Temperature regulation	Sample/stage: Delta T temperature controller Objective: Objective heater controller	Bioptechs, Inc.
7	Sample chambers	Delta T glass bottom dishes	Bioptechs, Inc.
8	High NA objective	U Apochromat 150X TIRF, NA 1.45	Olympus
9	Data acquisition software	IPLab	Scanalytics, Inc.
		SlideBook	Intelligent Imaging Innovations

Optional Devices
10	Autofocus	Built-in: IX81-ZDC	Olympus
		Retrofit: CRIFF-2000	Applied Scientific Instruments Inc.
11	High-speed shutters	Lambda DG4 and DG5	Sutter Instruments, Inc.
12	Automatic stage (x, y, and z positioning)	x, y, (and optional z) automatic servo stage (MS-2000) and controller with an additional linear encoder (800-706-2284) for more accurate reproducibility	Applied Scientific Instruments Inc.
13	Multichannel imaging device	Dual-View	Photometrics
		FW-1000 high-speed filter wheel	Applied Scientific Instruments Inc.
14	Digital micromirror array	Mosaic digital diaphragm system	Photonic Instruments, Inc.