Recipe

Nutrient-rich fly medium

Nutrient-rich fly medium

8 g agar

80 g cornmeal

18 g dried yeast

Live baker’s yeast

80 g malt extract

7 g molasses

6.3 mL propionic acid

10 g soybean meal

Dissolve ingredients (except propionic acid and live baker’s yeast) in 1 L of H2O. Boil extensively on a hot plate until all ingredients are well dissolved. Add the propionic acid (a mold inhibitor). Pour the medium into 175-mL bottles, and allow it to solidify. Add a large drop of live baker’s yeast on the surface of the medium in each bottle.

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  1. doi:10.1101/pdb.rec10915 Cold Spring Harb Protoc 2007: pdb.rec10915-

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