Recipe

B&E saline

Reagent Quantity (for 1 L)
NaCl 7.5 g
KCl 0.35 g
CaCl2 0.21 g
Tricine 1.793 g
Adjust pH to 6.9.
This recipe is adapted from Ephrussi and Beadle (1936).

REFERENCES

  1. ↵
    1. Ephrussi B.,
    2. Beadle G.W.
    (1936) A technique of transplantation for Drosophila. Am. Nat. 70:218–225.
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  1. doi:10.1101/pdb.rec11235 Cold Spring Harb Protoc 2007: pdb.rec11235-
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