Photograph and diagram of a Rose chamber used for imaging living cells. The photograph shows the assembled chamber; the coverslip with cells is on the bottom, and the chamber is used on an inverted microscope. The diagram shows the parts of the chamber. The upper and lower plates are aluminum, and the inner spacer is made by polymerizing a sheet of silastic elastomer of the desired thickness and cutting the openings using a cork borer. To prevent cracking the coverslip when the chamber is assembled, a piece of parafilm (with a central opening) can be placed over the lower plate before adding the coverslip. The entire assembly is held together with four screws, and a round coverslip is added as a lid on the top.