Recipe

Stock buffer

0.2 M Tris-HCl (pH 8.3)

2 mM EDTA

6 M guanidine-HCl

0.02% (w/v) Tween 20

Mix 1 volume of 1 M Tris-HCl (pH 8.3) and 5 volumes of aqueous 8 M guanidine-HCl. Adjust to 2 mM EDTA.

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  1. doi:10.1101/pdb.rec10639 Cold Spring Harb Protoc 2007: pdb.rec10639-
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