Recipe

Reduction buffer

0.2 M Tris-HCl, pH 8.5

2.5 mM EDTA

6 M guanidine-HCl

Urea (8 M) can be substituted for guanidine-HCl. Stock solutions of reduction buffer made with urea may be stored frozen for up to 2 weeks prior to use.

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  1. doi:10.1101/pdb.rec10643 Cold Spring Harb Protoc 2007: pdb.rec10643-
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