Recipe

Proteinase solution

0.1% SDS

10% glycerol

0.005% bromophenol blue

1-100 μg/ml proteinase in 0.125 m Tris-HCl (pH 6.8)

Endoproteinase Glu-C (or V8 protease), which can tolerate high concentrations of SDS, is recommended for the Cleveland method.

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  1. doi:10.1101/pdb.rec10645 Cold Spring Harb Protoc 2007: pdb.rec10645-
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