Recipe

2D equilibration solution

50 mM Tris-Cl (pH 6.8)

6 M urea

30% (v/v) glycerol

2% (w/v) SDS

2% (w/v) dithioerythritol (DTE)

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  1. doi:10.1101/pdb.rec10713 Cold Spring Harb Protoc 2007: pdb.rec10713-
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