Recipe

First-dimension sample loading buffer

8 M urea

4% (w/v) CHAPS

40 mM Tris

65 mM DTE

0.05% (w/v) SDS

A trace of bromophenol blue

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  1. doi:10.1101/pdb.rec10718 Cold Spring Harb Protoc 2007: pdb.rec10718-
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