Recipe

Overlay solution

0.5% (w/v) agarose

25 mM Tris (pH 8.3)

198 mM glycine

0.1% (w/v) SDS

Shortly before use, boil overlay solution to melt the agarose. Maintain the solution at ~70°C until use.

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  1. doi:10.1101/pdb.rec10720 Cold Spring Harb Protoc 2007: pdb.rec10720-
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