Lysis buffer for purification of interacting proteins

10 mM Tris-Cl (pH 7.5)

100 mM NaCl

0.5% Nonidet P-40

Immediately before use, add:

1 mM phenylmethylsulfonyl fluoride (PMSF)

Complete Protease Inhibitor Cocktail Tablets (Roche Applied Science)

1 mM sodium orthovanadate (Na₃VO₄)

1 mM sodium fluoride (NaF)