ACSF (Artificial cerebrospinal fluid)
124 mM NaCl
2.5 mM KCl
2.0 mM MgSO4
1.25 mM KH2PO4
26 mM NaHCO3
10 mM glucose
4 mM sucrose
When the components are first mixed, the ACSF solution may be slightly milky, but it should clear when aerated with 95% O2, 5% CO2, after which the pH of the medium should be 7.4.
