Recipe

Modified HEPES-buffered saline (MHBS)

135 mM NaCl

1 mM Na2HPO4

4 mM KCl

2 mM CaCl2

20 mM HEPES buffer (Sigma)

Make up 100 ml, adjust the pH to 7.5, and filter-sterilize.

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  1. doi:10.1101/pdb.rec10807 Cold Spring Harb Protoc 2007: pdb.rec10807-
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