Recipe

Assembly buffer I

6 mM sodium phosphate (see note below)

0.17 M NaCl

3 mM KCl

0.2% 2-mercaptoethanol

0.2 mM phenylmethylsulfonyl fluoride (PMSF)

Note: 6 mM potassium phosphate (pH 7.4) can be used in place of 6 mM sodium phosphate.

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  1. doi:10.1101/pdb.rec10828 Cold Spring Harb Protoc 2007: pdb.rec10828-
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