Recipe

Column buffer I

6 M urea

8 mM sodium phosphate (pH 7.2)

0.14 M NaCl

1 mM dithiothreitol (DTT)

0.1 mM phenylmethylsulfonyl fluoride (PMSF)

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  1. doi:10.1101/pdb.rec10829 Cold Spring Harb Protoc 2007: pdb.rec10829-
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