Recipe

Column buffer II

10 mM Tris-HCl (pH 8)

8 M urea

2 mM EDTA

10 mM DTT

To inhibit proteolysis, add the following protease inhibitors just before use:

1 mM PMSF

5 μg/ml pepstatin A

5 μg/ml leupeptin

5 μg/ml aprotinin

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  1. doi:10.1101/pdb.rec10830 Cold Spring Harb Protoc 2007: pdb.rec10830-
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