Recipe

Mannitol/MgCl2 solution

1 M Mannitol

100 mM MES-KOH (pH 5.6)

1 M MgCl2

Combine 600 μl of 1 M mannitol, 75 μl of 100 mM MES-KOH, and 22.5 μl of 1 M MgCl2. Add 800 μl of H2O.

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  1. doi:10.1101/pdb.rec10867 Cold Spring Harb Protoc 2007: pdb.rec10867-
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