Recipe

Protoplast wash solution

1 M CaCl2

1 M mannitol

Combine 400 μl of 1 M CaCl2 and 500 μl of 1 M mannitol in 2.1 ml of H2O.

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  1. doi:10.1101/pdb.rec10870 Cold Spring Harb Protoc 2007: pdb.rec10870-
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