Recipe

W5 solution

125 mM CaCl2

5 mM glucose

5 mM KCl

1.5 mM MES

154 mM NaCl

Adjust pH to 5.6 with KOH. Sterilize by either filtering or autoclaving.

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  1. doi:10.1101/pdb.rec10872 Cold Spring Harb Protoc 2007: pdb.rec10872-
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