| BNPS-skatole |
Trp-X |
Unstable under acidic conditions. Must be prepared fresh prior to use to minimize side reactions. |
| Cyanogen bromide (CNBr) |
Met-X |
Excellent specificity and yield. Cleavage of Met-Ser/Met-Thr bonds may give suboptimal yields. Excess CNBr can cleave/degrade
Trp and Tyr side-chains.
|
| Dilute acid (pH 2) |
Asp-X |
Yields >60%. Use formic acid for insoluble proteins. |
| DMSO/HCl/HBr |
Trp-X |
Oxidation and modification of amino acids likely. |
| Formic acid (80%) |
Asp-Pro |
Yields of 40% with high specificity. |
| HCl (6 M) |
Multiple |
For random generation of small peptides. |
| Hydroxylamine |
Asn-Gly |
Yields ~50%. Generally good specificity, although some Asn-Gly links are resistant and some Asn-X bonds may cleave. Also breaks
imide link to Gly.
|
| NTCB |
Cys-X |
Splits on the amino side; Cys amino blocked. |
| aCleavage occurs at the carboxyl end of the amino acid unless otherwise indicated.
|
