| Cyanogen bromide (CNBr) |
Met-X |
Blow nitrogen over the solution to displace oxygen, cap the tube, then incubate overnight at room temperature. |
| Hydroxylamine |
Asn-Gly |
Incubate mixture for 4 hr at 45°C. Stop the reaction by cooling the sample and adjusting the pH to 2.5 by the addition of
concentrated formic acid or 3 volumes of 2% (v/v) TFA in H2O.
|
| o-iodosobenzoic acid
|
Trp-X |
Add protein to a final concentration of 5-10 mg/ml. Flush the tube with nitrogen and incubate at room temperature for 24 hr
in the dark.
|
