Cleavage of Asn-Gly Bonds by Hydroxylamine
This protocol was adapted from “Peptide Mapping and Sequence Analysis of Gel-Resolved Proteins,” Chapter 7, in Proteins and Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.INTRODUCTION
Selective cleavage of Asn-Gly bonds in proteins by hydroxylamine generates a relatively small number of large peptide fragments. This protocol describes a procedure for performing Asn-Gly bond cleavage in solution.
