NHS solution

N,N-Diisopropylethylamine (5.73 M)

N,N-Dimethylformamide (DMF), anhydrous

N,N′-Disuccinimidyl carbonate

Dissolve 10.24 g of N,N′-disuccinimidyl carbonate and 6.96 ml of N,N-diisopropylethylamine in 400 ml of anhydrous DMF. Final concentrations of N,N′-disuccinimidyl carbonate and N,N-diisopropylethylamine are 100 mM each.