Lysis buffer for antibody arrays

Tris-Cl (20 mM, pH 7.5)

NaCl (150 mM)

BSA (1%, w/v)

EDTA (1 mM)

EGTA (1 mM)

Triton X-100 (1%)

Nonidet P-40 (0.5%)

β-glycerolphosphate (1 mM)

Sodium orthovanadate (Na₃VO₄) (1 mM)

PMSF (1 mM)

Leupeptin (1 μg/ml)

Pepstatin (1 μg/ml)