HEMG20

Prepare 25 ml for 50 g of Drosophila nuclei:

25 mM HEPES, potassium salt (pH 7.6)

0.1 M KCl

12.5 mM MgCl₂

0.1 mM EDTA

20% (v/v) glycerol

Store at 4ºC. Just before use, add the following (to 25 ml):

75 μl DTT (0.5 M)

50 μl sodium metabisulfite (0.5 M)

12.5 μl PMSF (0.2 M)

50 μl benzamidine (0.5 M)